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the human trim55 cdna  (Addgene inc)


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    Addgene inc the human trim55 cdna
    <t>TRIM55</t> expression was upregulated in HCC and associated with a poor prognosis in HCC patients. ( A ) TRIM55 mRNA expression in HCC tumor tissues and adjacent non-tumor tissues extracted from GSE84005, GSE64041, GSE39791 and GSE36376. ( B ) TRIM55 expression in HCC tumor tissues and adjacent non-tumor tissues in the TCGA database. ( C ) Representative immunohistochemical staining images of TRIM55 in 110 paired HCC tumor tissues and adjacent non-tumor tissues (Scale bars: 50 μm, 20 μm). ( D ) Statistical analysis of TRIM55 expression according to the immunohistochemistry results. ( E ) Kaplan-Meier overall survival curve of two HCC groups: high TRIM55 group: n = 67; low TRIM55 group: n = 43. ( F ) Kaplan-Meier disease-free survival curve of two HCC groups: high TRIM55 group: n = 67; low TRIM55 group: n = 43. (*P < 0.05, **P < 0.01, ****P < 0.0001).
    The Human Trim55 Cdna, supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/the+human+trim55+cdna/pmc10406114-45-2-19?v=Addgene+inc
    Average 90 stars, based on 1 article reviews
    the human trim55 cdna - by Bioz Stars, 2026-07
    90/100 stars

    Images

    1) Product Images from "TRIM55 Promotes Proliferation of Hepatocellular Carcinoma Through Stabilizing TRIP6 to Activate Wnt/β-Catenin Signaling"

    Article Title: TRIM55 Promotes Proliferation of Hepatocellular Carcinoma Through Stabilizing TRIP6 to Activate Wnt/β-Catenin Signaling

    Journal: Journal of Hepatocellular Carcinoma

    doi: 10.2147/JHC.S418049

    TRIM55 expression was upregulated in HCC and associated with a poor prognosis in HCC patients. ( A ) TRIM55 mRNA expression in HCC tumor tissues and adjacent non-tumor tissues extracted from GSE84005, GSE64041, GSE39791 and GSE36376. ( B ) TRIM55 expression in HCC tumor tissues and adjacent non-tumor tissues in the TCGA database. ( C ) Representative immunohistochemical staining images of TRIM55 in 110 paired HCC tumor tissues and adjacent non-tumor tissues (Scale bars: 50 μm, 20 μm). ( D ) Statistical analysis of TRIM55 expression according to the immunohistochemistry results. ( E ) Kaplan-Meier overall survival curve of two HCC groups: high TRIM55 group: n = 67; low TRIM55 group: n = 43. ( F ) Kaplan-Meier disease-free survival curve of two HCC groups: high TRIM55 group: n = 67; low TRIM55 group: n = 43. (*P < 0.05, **P < 0.01, ****P < 0.0001).
    Figure Legend Snippet: TRIM55 expression was upregulated in HCC and associated with a poor prognosis in HCC patients. ( A ) TRIM55 mRNA expression in HCC tumor tissues and adjacent non-tumor tissues extracted from GSE84005, GSE64041, GSE39791 and GSE36376. ( B ) TRIM55 expression in HCC tumor tissues and adjacent non-tumor tissues in the TCGA database. ( C ) Representative immunohistochemical staining images of TRIM55 in 110 paired HCC tumor tissues and adjacent non-tumor tissues (Scale bars: 50 μm, 20 μm). ( D ) Statistical analysis of TRIM55 expression according to the immunohistochemistry results. ( E ) Kaplan-Meier overall survival curve of two HCC groups: high TRIM55 group: n = 67; low TRIM55 group: n = 43. ( F ) Kaplan-Meier disease-free survival curve of two HCC groups: high TRIM55 group: n = 67; low TRIM55 group: n = 43. (*P < 0.05, **P < 0.01, ****P < 0.0001).

    Techniques Used: Expressing, Immunohistochemical staining, Staining, Immunohistochemistry

    TRIM55 promotes HCC cell proliferation in vitro and in vivo. ( A ) The overexpression of TRIM55 in HLF and HepG2 cells were verified by Western blot analysis. ( B ) Knockdown of TRIM55 in 97H and Alex cells were verified by Western blot analysis. ( C and D ) The effect of TRIM55 overexpression or knockdown on cell proliferation evaluated by CCK8 assay. ( E and F ) The effect of TRIM55 overexpression or knockdown on cell proliferation evaluated by colony formation assay. ( G and H ) The effect of TRIM55 overexpression or knockdown on growth of xenograft tumors. Tumor growth was evaluated by measuring tumor volume. ( I and J ) Representative images of the effect of TRIM55 overexpression or knockdown on growth of intrahepatic tumors. Tumor growth was evaluated by measuring tumor volume. ( K and L ) Representative immunohistochemical images of expression level of Ki-67 in xenograft tumor tissues. (*P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001.).
    Figure Legend Snippet: TRIM55 promotes HCC cell proliferation in vitro and in vivo. ( A ) The overexpression of TRIM55 in HLF and HepG2 cells were verified by Western blot analysis. ( B ) Knockdown of TRIM55 in 97H and Alex cells were verified by Western blot analysis. ( C and D ) The effect of TRIM55 overexpression or knockdown on cell proliferation evaluated by CCK8 assay. ( E and F ) The effect of TRIM55 overexpression or knockdown on cell proliferation evaluated by colony formation assay. ( G and H ) The effect of TRIM55 overexpression or knockdown on growth of xenograft tumors. Tumor growth was evaluated by measuring tumor volume. ( I and J ) Representative images of the effect of TRIM55 overexpression or knockdown on growth of intrahepatic tumors. Tumor growth was evaluated by measuring tumor volume. ( K and L ) Representative immunohistochemical images of expression level of Ki-67 in xenograft tumor tissues. (*P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001.).

    Techniques Used: In Vitro, In Vivo, Over Expression, Western Blot, CCK-8 Assay, Colony Assay, Immunohistochemical staining, Expressing

    TRIM55 interacts and colocalizes with TRIP6. ( A ) Proteins associated with TRIM55 were identified by mass spectrometry. ( B ) The TRIP6 peptides identified by mass spectrometry were shown. ( C ) The interaction between Flag-TRIM55 and HA-TRIP6 was analyzed by Co-IP in co-transfected HEK293T cells. ( D ) The interaction between endogenous TRIM55 and TRIP6 was analyzed by Co-IP in HLF and HepG2 cells with TRIM55-overexpression. ( E ) Confocal assays confirmed that TRIM55 and TRIP6 were mainly co-localized in the cytoplasm (Scale bar: 10 μm, 63 X objective).
    Figure Legend Snippet: TRIM55 interacts and colocalizes with TRIP6. ( A ) Proteins associated with TRIM55 were identified by mass spectrometry. ( B ) The TRIP6 peptides identified by mass spectrometry were shown. ( C ) The interaction between Flag-TRIM55 and HA-TRIP6 was analyzed by Co-IP in co-transfected HEK293T cells. ( D ) The interaction between endogenous TRIM55 and TRIP6 was analyzed by Co-IP in HLF and HepG2 cells with TRIM55-overexpression. ( E ) Confocal assays confirmed that TRIM55 and TRIP6 were mainly co-localized in the cytoplasm (Scale bar: 10 μm, 63 X objective).

    Techniques Used: Mass Spectrometry, Co-Immunoprecipitation Assay, Transfection, Over Expression

    Tumor promoting effect of TRIM55 in HCC is partially dependent on TRIP6. ( A ) The effect of TRIM55 overexpression or knockdown on TRIP6, β-Catenin and Cyclin D1 protein levels by Western blot analysis. ( B ) The effect of TRIM55 overexpression or knockdown on Wnt activity by TOP/FOP luciferase reporter assay. ( C ) Knockdown of TRIP6 in HLF and HepG2 cells with TRIM55 overexpression was verified by Western blot analysis. ( D ) CCK8 assay was performed in HLF and HepG2 cells with TRIM55-overexpression or control cells transiently transfected with siNC, siTRIP6. ( E ) Colony formation assay was performed in HLF and HepG2 cells with TRIM55-overexpression or control cells transiently transfected with siNC, siTRIP6. ( F ) TRIP6 knockdown significantly decreased the TOP/FOP luciferase activity induced by TRIM55. ( G ) Western blot analysis of TRIP6, β-Catenin and Cyclin D1 protein levels in HLF and HepG2 cells with TRIM55-overexpression or control cells transiently transfected with siNC, siTRIP6. (*P < 0.05, **P < 0.01, ***P < 0.001).
    Figure Legend Snippet: Tumor promoting effect of TRIM55 in HCC is partially dependent on TRIP6. ( A ) The effect of TRIM55 overexpression or knockdown on TRIP6, β-Catenin and Cyclin D1 protein levels by Western blot analysis. ( B ) The effect of TRIM55 overexpression or knockdown on Wnt activity by TOP/FOP luciferase reporter assay. ( C ) Knockdown of TRIP6 in HLF and HepG2 cells with TRIM55 overexpression was verified by Western blot analysis. ( D ) CCK8 assay was performed in HLF and HepG2 cells with TRIM55-overexpression or control cells transiently transfected with siNC, siTRIP6. ( E ) Colony formation assay was performed in HLF and HepG2 cells with TRIM55-overexpression or control cells transiently transfected with siNC, siTRIP6. ( F ) TRIP6 knockdown significantly decreased the TOP/FOP luciferase activity induced by TRIM55. ( G ) Western blot analysis of TRIP6, β-Catenin and Cyclin D1 protein levels in HLF and HepG2 cells with TRIM55-overexpression or control cells transiently transfected with siNC, siTRIP6. (*P < 0.05, **P < 0.01, ***P < 0.001).

    Techniques Used: Over Expression, Western Blot, Activity Assay, Luciferase, Reporter Assay, CCK-8 Assay, Transfection, Colony Assay

    TRIM55 stabilizes TRIP6 protein by inhibiting K48-linked ubiquitination and inducing K63-linked ubiquitination. ( A ) The effects of transfection of Flag-TRIM55 with different concentrations on HA-TRIP6 (0.5μg) were detected in HEK293T cells by Western blot assay. ( B and C ) HLF-TRIM55 and HepG2-TRIM55 or control cells were treated with CHX (10 μM) for 0, 2, 4 and 8 h, the levels of TRIP6 were detected by Western blot assay. Quantification of the TRIP6 levels relative to GAPDH expression is shown. ( D and E ) HLF-TRIM55 and HepG2-TRIM55 or control cells were treated with Mg132 (20 μM) for 6 h, and the ubiquitination of TRIP6 was detected by immunoprecipitation (IP). ( F ) Co-IP analysis of ubiquitination of TRIP6 in 293T cells co-transfected with HA-TRIP6, Flag-TRIM55 and Myc-Ub plasmids. ( G and H ) Co-IP analysis of ubiquitination of TRIP6 in 293T cells co-transfected with Flag-TRIM55, Myc -TRIP6, HA-Ub-K48 or HA-Ub-K63 plasmids.
    Figure Legend Snippet: TRIM55 stabilizes TRIP6 protein by inhibiting K48-linked ubiquitination and inducing K63-linked ubiquitination. ( A ) The effects of transfection of Flag-TRIM55 with different concentrations on HA-TRIP6 (0.5μg) were detected in HEK293T cells by Western blot assay. ( B and C ) HLF-TRIM55 and HepG2-TRIM55 or control cells were treated with CHX (10 μM) for 0, 2, 4 and 8 h, the levels of TRIP6 were detected by Western blot assay. Quantification of the TRIP6 levels relative to GAPDH expression is shown. ( D and E ) HLF-TRIM55 and HepG2-TRIM55 or control cells were treated with Mg132 (20 μM) for 6 h, and the ubiquitination of TRIP6 was detected by immunoprecipitation (IP). ( F ) Co-IP analysis of ubiquitination of TRIP6 in 293T cells co-transfected with HA-TRIP6, Flag-TRIM55 and Myc-Ub plasmids. ( G and H ) Co-IP analysis of ubiquitination of TRIP6 in 293T cells co-transfected with Flag-TRIM55, Myc -TRIP6, HA-Ub-K48 or HA-Ub-K63 plasmids.

    Techniques Used: Transfection, Western Blot, Expressing, Immunoprecipitation, Co-Immunoprecipitation Assay

    Expression of TRIM55 and TRIP6 were positively correlated in human HCC tissues. ( A ) Representative IHC staining figures of TRIM55 and TRIP6 expression in serial sections of HCC tumor samples (Scale bar: 50 μm, 20 μm; 20 X, 40 X objective). ( B ) Distribution of TRIP6 expression level in high TRIM55 expression level group and low TRIM55 expression level group tissues. ( C ) Pearson correlation analysis between TRIM55 and TRIP6 expression, N = 110. (*P < 0.05).
    Figure Legend Snippet: Expression of TRIM55 and TRIP6 were positively correlated in human HCC tissues. ( A ) Representative IHC staining figures of TRIM55 and TRIP6 expression in serial sections of HCC tumor samples (Scale bar: 50 μm, 20 μm; 20 X, 40 X objective). ( B ) Distribution of TRIP6 expression level in high TRIM55 expression level group and low TRIM55 expression level group tissues. ( C ) Pearson correlation analysis between TRIM55 and TRIP6 expression, N = 110. (*P < 0.05).

    Techniques Used: Expressing, Immunohistochemistry



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    the human trim55 cdna  (Addgene inc)
    90
    Addgene inc the human trim55 cdna
    <t>TRIM55</t> expression was upregulated in HCC and associated with a poor prognosis in HCC patients. ( A ) TRIM55 mRNA expression in HCC tumor tissues and adjacent non-tumor tissues extracted from GSE84005, GSE64041, GSE39791 and GSE36376. ( B ) TRIM55 expression in HCC tumor tissues and adjacent non-tumor tissues in the TCGA database. ( C ) Representative immunohistochemical staining images of TRIM55 in 110 paired HCC tumor tissues and adjacent non-tumor tissues (Scale bars: 50 μm, 20 μm). ( D ) Statistical analysis of TRIM55 expression according to the immunohistochemistry results. ( E ) Kaplan-Meier overall survival curve of two HCC groups: high TRIM55 group: n = 67; low TRIM55 group: n = 43. ( F ) Kaplan-Meier disease-free survival curve of two HCC groups: high TRIM55 group: n = 67; low TRIM55 group: n = 43. (*P < 0.05, **P < 0.01, ****P < 0.0001).
    The Human Trim55 Cdna, supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/the+human+trim55+cdna/pmc10406114-45-2-19?v=Addgene+inc
    Average 90 stars, based on 1 article reviews
    the human trim55 cdna - by Bioz Stars, 2026-07
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    TRIM55 expression was upregulated in HCC and associated with a poor prognosis in HCC patients. ( A ) TRIM55 mRNA expression in HCC tumor tissues and adjacent non-tumor tissues extracted from GSE84005, GSE64041, GSE39791 and GSE36376. ( B ) TRIM55 expression in HCC tumor tissues and adjacent non-tumor tissues in the TCGA database. ( C ) Representative immunohistochemical staining images of TRIM55 in 110 paired HCC tumor tissues and adjacent non-tumor tissues (Scale bars: 50 μm, 20 μm). ( D ) Statistical analysis of TRIM55 expression according to the immunohistochemistry results. ( E ) Kaplan-Meier overall survival curve of two HCC groups: high TRIM55 group: n = 67; low TRIM55 group: n = 43. ( F ) Kaplan-Meier disease-free survival curve of two HCC groups: high TRIM55 group: n = 67; low TRIM55 group: n = 43. (*P < 0.05, **P < 0.01, ****P < 0.0001).

    Journal: Journal of Hepatocellular Carcinoma

    Article Title: TRIM55 Promotes Proliferation of Hepatocellular Carcinoma Through Stabilizing TRIP6 to Activate Wnt/β-Catenin Signaling

    doi: 10.2147/JHC.S418049

    Figure Lengend Snippet: TRIM55 expression was upregulated in HCC and associated with a poor prognosis in HCC patients. ( A ) TRIM55 mRNA expression in HCC tumor tissues and adjacent non-tumor tissues extracted from GSE84005, GSE64041, GSE39791 and GSE36376. ( B ) TRIM55 expression in HCC tumor tissues and adjacent non-tumor tissues in the TCGA database. ( C ) Representative immunohistochemical staining images of TRIM55 in 110 paired HCC tumor tissues and adjacent non-tumor tissues (Scale bars: 50 μm, 20 μm). ( D ) Statistical analysis of TRIM55 expression according to the immunohistochemistry results. ( E ) Kaplan-Meier overall survival curve of two HCC groups: high TRIM55 group: n = 67; low TRIM55 group: n = 43. ( F ) Kaplan-Meier disease-free survival curve of two HCC groups: high TRIM55 group: n = 67; low TRIM55 group: n = 43. (*P < 0.05, **P < 0.01, ****P < 0.0001).

    Article Snippet: To construct TRIM55 overexpressing cell lines, the human TRIM55 cDNA and negative control were cloned into the pLenti-CMV-Puro plasmid (Addgene #17448).

    Techniques: Expressing, Immunohistochemical staining, Staining, Immunohistochemistry

    TRIM55 promotes HCC cell proliferation in vitro and in vivo. ( A ) The overexpression of TRIM55 in HLF and HepG2 cells were verified by Western blot analysis. ( B ) Knockdown of TRIM55 in 97H and Alex cells were verified by Western blot analysis. ( C and D ) The effect of TRIM55 overexpression or knockdown on cell proliferation evaluated by CCK8 assay. ( E and F ) The effect of TRIM55 overexpression or knockdown on cell proliferation evaluated by colony formation assay. ( G and H ) The effect of TRIM55 overexpression or knockdown on growth of xenograft tumors. Tumor growth was evaluated by measuring tumor volume. ( I and J ) Representative images of the effect of TRIM55 overexpression or knockdown on growth of intrahepatic tumors. Tumor growth was evaluated by measuring tumor volume. ( K and L ) Representative immunohistochemical images of expression level of Ki-67 in xenograft tumor tissues. (*P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001.).

    Journal: Journal of Hepatocellular Carcinoma

    Article Title: TRIM55 Promotes Proliferation of Hepatocellular Carcinoma Through Stabilizing TRIP6 to Activate Wnt/β-Catenin Signaling

    doi: 10.2147/JHC.S418049

    Figure Lengend Snippet: TRIM55 promotes HCC cell proliferation in vitro and in vivo. ( A ) The overexpression of TRIM55 in HLF and HepG2 cells were verified by Western blot analysis. ( B ) Knockdown of TRIM55 in 97H and Alex cells were verified by Western blot analysis. ( C and D ) The effect of TRIM55 overexpression or knockdown on cell proliferation evaluated by CCK8 assay. ( E and F ) The effect of TRIM55 overexpression or knockdown on cell proliferation evaluated by colony formation assay. ( G and H ) The effect of TRIM55 overexpression or knockdown on growth of xenograft tumors. Tumor growth was evaluated by measuring tumor volume. ( I and J ) Representative images of the effect of TRIM55 overexpression or knockdown on growth of intrahepatic tumors. Tumor growth was evaluated by measuring tumor volume. ( K and L ) Representative immunohistochemical images of expression level of Ki-67 in xenograft tumor tissues. (*P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001.).

    Article Snippet: To construct TRIM55 overexpressing cell lines, the human TRIM55 cDNA and negative control were cloned into the pLenti-CMV-Puro plasmid (Addgene #17448).

    Techniques: In Vitro, In Vivo, Over Expression, Western Blot, CCK-8 Assay, Colony Assay, Immunohistochemical staining, Expressing

    TRIM55 interacts and colocalizes with TRIP6. ( A ) Proteins associated with TRIM55 were identified by mass spectrometry. ( B ) The TRIP6 peptides identified by mass spectrometry were shown. ( C ) The interaction between Flag-TRIM55 and HA-TRIP6 was analyzed by Co-IP in co-transfected HEK293T cells. ( D ) The interaction between endogenous TRIM55 and TRIP6 was analyzed by Co-IP in HLF and HepG2 cells with TRIM55-overexpression. ( E ) Confocal assays confirmed that TRIM55 and TRIP6 were mainly co-localized in the cytoplasm (Scale bar: 10 μm, 63 X objective).

    Journal: Journal of Hepatocellular Carcinoma

    Article Title: TRIM55 Promotes Proliferation of Hepatocellular Carcinoma Through Stabilizing TRIP6 to Activate Wnt/β-Catenin Signaling

    doi: 10.2147/JHC.S418049

    Figure Lengend Snippet: TRIM55 interacts and colocalizes with TRIP6. ( A ) Proteins associated with TRIM55 were identified by mass spectrometry. ( B ) The TRIP6 peptides identified by mass spectrometry were shown. ( C ) The interaction between Flag-TRIM55 and HA-TRIP6 was analyzed by Co-IP in co-transfected HEK293T cells. ( D ) The interaction between endogenous TRIM55 and TRIP6 was analyzed by Co-IP in HLF and HepG2 cells with TRIM55-overexpression. ( E ) Confocal assays confirmed that TRIM55 and TRIP6 were mainly co-localized in the cytoplasm (Scale bar: 10 μm, 63 X objective).

    Article Snippet: To construct TRIM55 overexpressing cell lines, the human TRIM55 cDNA and negative control were cloned into the pLenti-CMV-Puro plasmid (Addgene #17448).

    Techniques: Mass Spectrometry, Co-Immunoprecipitation Assay, Transfection, Over Expression

    Tumor promoting effect of TRIM55 in HCC is partially dependent on TRIP6. ( A ) The effect of TRIM55 overexpression or knockdown on TRIP6, β-Catenin and Cyclin D1 protein levels by Western blot analysis. ( B ) The effect of TRIM55 overexpression or knockdown on Wnt activity by TOP/FOP luciferase reporter assay. ( C ) Knockdown of TRIP6 in HLF and HepG2 cells with TRIM55 overexpression was verified by Western blot analysis. ( D ) CCK8 assay was performed in HLF and HepG2 cells with TRIM55-overexpression or control cells transiently transfected with siNC, siTRIP6. ( E ) Colony formation assay was performed in HLF and HepG2 cells with TRIM55-overexpression or control cells transiently transfected with siNC, siTRIP6. ( F ) TRIP6 knockdown significantly decreased the TOP/FOP luciferase activity induced by TRIM55. ( G ) Western blot analysis of TRIP6, β-Catenin and Cyclin D1 protein levels in HLF and HepG2 cells with TRIM55-overexpression or control cells transiently transfected with siNC, siTRIP6. (*P < 0.05, **P < 0.01, ***P < 0.001).

    Journal: Journal of Hepatocellular Carcinoma

    Article Title: TRIM55 Promotes Proliferation of Hepatocellular Carcinoma Through Stabilizing TRIP6 to Activate Wnt/β-Catenin Signaling

    doi: 10.2147/JHC.S418049

    Figure Lengend Snippet: Tumor promoting effect of TRIM55 in HCC is partially dependent on TRIP6. ( A ) The effect of TRIM55 overexpression or knockdown on TRIP6, β-Catenin and Cyclin D1 protein levels by Western blot analysis. ( B ) The effect of TRIM55 overexpression or knockdown on Wnt activity by TOP/FOP luciferase reporter assay. ( C ) Knockdown of TRIP6 in HLF and HepG2 cells with TRIM55 overexpression was verified by Western blot analysis. ( D ) CCK8 assay was performed in HLF and HepG2 cells with TRIM55-overexpression or control cells transiently transfected with siNC, siTRIP6. ( E ) Colony formation assay was performed in HLF and HepG2 cells with TRIM55-overexpression or control cells transiently transfected with siNC, siTRIP6. ( F ) TRIP6 knockdown significantly decreased the TOP/FOP luciferase activity induced by TRIM55. ( G ) Western blot analysis of TRIP6, β-Catenin and Cyclin D1 protein levels in HLF and HepG2 cells with TRIM55-overexpression or control cells transiently transfected with siNC, siTRIP6. (*P < 0.05, **P < 0.01, ***P < 0.001).

    Article Snippet: To construct TRIM55 overexpressing cell lines, the human TRIM55 cDNA and negative control were cloned into the pLenti-CMV-Puro plasmid (Addgene #17448).

    Techniques: Over Expression, Western Blot, Activity Assay, Luciferase, Reporter Assay, CCK-8 Assay, Transfection, Colony Assay

    TRIM55 stabilizes TRIP6 protein by inhibiting K48-linked ubiquitination and inducing K63-linked ubiquitination. ( A ) The effects of transfection of Flag-TRIM55 with different concentrations on HA-TRIP6 (0.5μg) were detected in HEK293T cells by Western blot assay. ( B and C ) HLF-TRIM55 and HepG2-TRIM55 or control cells were treated with CHX (10 μM) for 0, 2, 4 and 8 h, the levels of TRIP6 were detected by Western blot assay. Quantification of the TRIP6 levels relative to GAPDH expression is shown. ( D and E ) HLF-TRIM55 and HepG2-TRIM55 or control cells were treated with Mg132 (20 μM) for 6 h, and the ubiquitination of TRIP6 was detected by immunoprecipitation (IP). ( F ) Co-IP analysis of ubiquitination of TRIP6 in 293T cells co-transfected with HA-TRIP6, Flag-TRIM55 and Myc-Ub plasmids. ( G and H ) Co-IP analysis of ubiquitination of TRIP6 in 293T cells co-transfected with Flag-TRIM55, Myc -TRIP6, HA-Ub-K48 or HA-Ub-K63 plasmids.

    Journal: Journal of Hepatocellular Carcinoma

    Article Title: TRIM55 Promotes Proliferation of Hepatocellular Carcinoma Through Stabilizing TRIP6 to Activate Wnt/β-Catenin Signaling

    doi: 10.2147/JHC.S418049

    Figure Lengend Snippet: TRIM55 stabilizes TRIP6 protein by inhibiting K48-linked ubiquitination and inducing K63-linked ubiquitination. ( A ) The effects of transfection of Flag-TRIM55 with different concentrations on HA-TRIP6 (0.5μg) were detected in HEK293T cells by Western blot assay. ( B and C ) HLF-TRIM55 and HepG2-TRIM55 or control cells were treated with CHX (10 μM) for 0, 2, 4 and 8 h, the levels of TRIP6 were detected by Western blot assay. Quantification of the TRIP6 levels relative to GAPDH expression is shown. ( D and E ) HLF-TRIM55 and HepG2-TRIM55 or control cells were treated with Mg132 (20 μM) for 6 h, and the ubiquitination of TRIP6 was detected by immunoprecipitation (IP). ( F ) Co-IP analysis of ubiquitination of TRIP6 in 293T cells co-transfected with HA-TRIP6, Flag-TRIM55 and Myc-Ub plasmids. ( G and H ) Co-IP analysis of ubiquitination of TRIP6 in 293T cells co-transfected with Flag-TRIM55, Myc -TRIP6, HA-Ub-K48 or HA-Ub-K63 plasmids.

    Article Snippet: To construct TRIM55 overexpressing cell lines, the human TRIM55 cDNA and negative control were cloned into the pLenti-CMV-Puro plasmid (Addgene #17448).

    Techniques: Transfection, Western Blot, Expressing, Immunoprecipitation, Co-Immunoprecipitation Assay

    Expression of TRIM55 and TRIP6 were positively correlated in human HCC tissues. ( A ) Representative IHC staining figures of TRIM55 and TRIP6 expression in serial sections of HCC tumor samples (Scale bar: 50 μm, 20 μm; 20 X, 40 X objective). ( B ) Distribution of TRIP6 expression level in high TRIM55 expression level group and low TRIM55 expression level group tissues. ( C ) Pearson correlation analysis between TRIM55 and TRIP6 expression, N = 110. (*P < 0.05).

    Journal: Journal of Hepatocellular Carcinoma

    Article Title: TRIM55 Promotes Proliferation of Hepatocellular Carcinoma Through Stabilizing TRIP6 to Activate Wnt/β-Catenin Signaling

    doi: 10.2147/JHC.S418049

    Figure Lengend Snippet: Expression of TRIM55 and TRIP6 were positively correlated in human HCC tissues. ( A ) Representative IHC staining figures of TRIM55 and TRIP6 expression in serial sections of HCC tumor samples (Scale bar: 50 μm, 20 μm; 20 X, 40 X objective). ( B ) Distribution of TRIP6 expression level in high TRIM55 expression level group and low TRIM55 expression level group tissues. ( C ) Pearson correlation analysis between TRIM55 and TRIP6 expression, N = 110. (*P < 0.05).

    Article Snippet: To construct TRIM55 overexpressing cell lines, the human TRIM55 cDNA and negative control were cloned into the pLenti-CMV-Puro plasmid (Addgene #17448).

    Techniques: Expressing, Immunohistochemistry